A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Brazilian Journal of Microbiology

Gavish Kumar²  Hari Shankar²  Deepa Bisht¹  Prashant Sharma¹  Neelja Singhal¹  V.m. Katoch¹  Beenu Joshi² 

[1] ,National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology Agra,India

关键词: Culture filtrate proteins;    M. tuberculosis;    SDS-TCA procedure;    Two-dimensional gel electrophoresis;   

DOI  :  10.1590/S1517-83822010000200005

来源: SciELO

【 摘 要 】

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.

【 授权许可】

CC BY-NC   
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