I.Structure-function analysis of the mechanosensitive channel of large conductance.II.Design of novel magnetic materials using crystal engineering

E. coli;ion-channel;M. tuberculosis;mechanosensitive channel of large conductance;Molecular Magnetism;MscL;salen

Maurer, Joshua Ahab ; Dougherty, Dennis A.

University:California Institute of Technology

Department:Chemistry and Chemical Engineering

关键词: E. coli;    ion-channel;    M. tuberculosis;    mechanosensitive channel of large conductance;    Molecular Magnetism;    MscL;    salen;   

Others  :  https://thesis.library.caltech.edu/4188/1/appendix_I.doc.pdf

美国|英语

来源: Caltech THESIS

【 摘 要 】

The work presented here encompasses two distinct areas, with the first section addressing structure-function relationships in the mechanosensitive channel of large conductance (MscL) from bacteria. A high-throughput fluorescent screening technique has been developed for the E. coli homologue of MscL.This technique has been applied to a large library of random E. coli MscL mutations to provide insights into channel function.Additionally, attempts have been made to characterize the functionally important regions of MscL and comparisons have been made between the E. coli and M. tuberculosis homologues of MscL.The second section addresses the design of novel magnetic materials.The guanidinium sulfonate ?Ward lattice? from crystal engineering has been used to develop a new family of frustrated magnetic materials.