The influence of mutation gene rpoB of Mycobacterium tuberculosis cluster I (507-534) on the elimination 25-desacetyl rifampicin in urine of tuberculosis subjects

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Lily^1 ; Sinaga, B.Y.M.^2 ; Ardinata, D.^3 ; Siregar, Y.^4

Master of Biomedical Sciences, Faculty of Medicine, Universitas Sumatera Utara, Medan

20155, Indonesia^1

Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sumatera Utara, RSUP. Haji Adam Malik, Medan

20136, Indonesia^2

Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan

20155, Indonesia^3

Department of Biomedical Sciences, Faculty of Medicine, Universitas Sumatera Utara, Medan

20155, Indonesia^4

关键词: Confidence interval;    Distributed data;    Fixed-dose combination;    M. tuberculosis;    Mycobacterium tuberculosis;    P-values;    Shapiro-Wilk tests;    Standard deviation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012146/pdf
DOI  :  10.1088/1755-1315/125/1/012146

来源: IOP

【 摘 要 】

The study aimed to evaluate the influence of mutation gene rpoB of Mycobacterium (M.) tuberculosis cluster I (507-534) on the elimination of 25-Desacetyl Rifampicin (25-DR) in the urine of tuberculosis (TB) subjects. Early morning sputum took from patient TB before treatment. Urine collected after 2 hours taken Fixed-Dose Combination (FDC) at days 7thtreatment. All sputum were sequencing at Macrogen Korea Laboratory. Urine was analyzed by high-Performance Liquid Chromatography (HPLC) using the method of Lily et al. Mean (standard deviation) for mutation and non-mutation of rpoB M. tuberculosis group were 7.6147 (4.4478) and 4.5772 (1.7532) μg/ml, respectively. Shapiro-Wilk test showed normally distributed data, with significance 0.3. Independent t-test performed p-value 0.167 and confidence interval (CI) from -1.648 to 7.723. The mutation gene rpoB of M. tuberculosis cluster, I (507-534) in this study, did not affect elimination 25-DR in theurine of TB subjects statistically and clinically.