Comparative study between the effects of hyaluronic acid and acid galactan purified from eggs of the mollusk Pomacea sp in wound healing

Acta Cirurgica Brasileira

Ana Katarina Menezes Da Cruz²  Wogelsanger Oliveira Pereira²  Elizeu Antunes Dos Santos²  Maria Goretti Freire Carvalho¹  Aldo Da Cunha Medeiros¹  Fernanda Wanderley De Oliveira² 

[1] ,Universidade Federal do Rio Grande do Norte Department of Biochemistry Natal

关键词: Acid galactan;    Hyaluronic acid;    Pomacea sp;    Glycosaminoglycans;    Galactano acídico;    Ácido hialurônico;    Pomacea sp;    Glicosaminoglicanos;   

DOI  :  10.1590/S0102-86502004000100002

来源: SciELO

【 摘 要 】

PURPOSE: To compare the effect of hyaluronic acid (HA) and of AG on the healing of intestine wounds. METHODS: The semi-purified extract of the eggs of the mollusc was obtained by fractionation with ammonium sulfate and purification for ion-exchange chromatography. The obtained galactans were eluted in water (neutral galactan) and in 0.1 and 0.2M NaCl (acidic galactans). The in vivo study was performed with 45 "Wistar" rats, separated in three groups (n=15). Solutions containing HA 1%, GA 1% or saline solution 0,9%, was placed topically on the sutures of wounds in the small intestine of the rats. After 05, 10 and 21 days the animals were sacrificed and biopsy of the healing tissue was done. RESULTS: The hystologic grading was more significant for HA and AG groups when compared to the group C. AG stimulated the appearance of macrophages, giant cells and increase in the concentration of collagen in the area of the wound when compared to HA. CONCLUSION: The topical use of GA in intestinal wounds promoted the anticipation of events that are important in the wound healing.

【 授权许可】

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