Glycosaminoglycans exposed on the endothelial cell surface

FEBS Letters

Vannucchi, Simonetta¹  Cavari, Simone¹ 

[1] Istituto di Patologia Generale, University of Firenze, Viale Morgagni 50, 50 134 Firenze, Italy

关键词: Heparin;    Glycosaminoglycans;    Endothelial cell;   

DOI  :  10.1016/0014-5793(93)81469-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the glycosaminoglycans exposed on the surface of endothelial cells cultured in vitro. The cells were labelled with [³⁵S]sodium sulfate. Chondroitin sulfate, heparan sulfate and dermatan sulfate are the main sulfated glycosaminoglycans present on the cell surface. They are synthesized by endothelial cells. However, the electrophoretic analysis and corresponding autoradiography of the glycosaminoglycans removed from the endothelial cell surface shows the presence of heparin-like compound which is not synthesized by the cells as it is unlabelled by [³⁵S]sodium sulfate. We show here that a proteolytic treatment of the commonly used serum for cell culturing, reveals the presence of an heparin-like anticoagulant activity. These results suggest that the endothelial cells bind endogenous heparin-like molecules present in serum.

【 授权许可】

Unknown