Effects of a 15-month anti-TNF-α treatment on plasma levels of glycosaminoglycans in women with rheumatoid arthritis

Arthritis Research & Therapy

Agnieszka Jura-Półtorak¹  Anna Szeremeta¹  Krystyna Olczyk¹  Ewa Maria Koźma¹  Andrzej Głowacki¹  Eugeniusz Józef Kucharz²  Magdalena Kopeć-Mędrek² 

[1] Department of Clinical Chemistry and Laboratory Diagnostics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice;Department of Internal Medicine and Rheumatology, School of Medicine in Katowice, Medical University of Silesia in Katowice;

关键词: Rheumatoid arthritis;    Tumor necrosis factor-alpha inhibitors;    Glycosaminoglycans;    Keratan sulfate;    Hyaluronic acid;   

DOI  :  10.1186/s13075-018-1711-z

来源: DOAJ

【 摘 要 】

Abstract Background In this study, the effect of 15-month anti-tumor necrosis factor alpha (TNF-α) treatment on circulating levels of plasma sulfated glycosaminoglycans (GAGs) and the nonsulfated GAG hyaluronic acid (HA) in female rheumatoid arthritis (RA) patients was assessed. Methods Plasma was obtained from healthy subjects and RA women treated with TNF-α antagonists (etanercept or adalimumab or certolizumab pegol) in combination with methotrexate. GAGs were isolated from plasma samples using ion exchange low-pressure liquid chromatography. Total sulfated GAGs were quantified using a hexuronic acid assay. Plasma levels of keratan sulfate (KS) and HA were measured using immunoassay kits. Results Total sulfated GAGs and HA levels were higher in female RA patients before treatment in comparison to healthy subjects. KS levels did not differ between RA women and controls. Anti-TNF-α treatment resulted in normalization of plasma total GAG and HA levels in RA patients, without any effect on KS levels. Conclusions Our results suggest that anti-TNF-α therapy has a beneficial effect on extracellular matrix remodeling in the course of RA.

【 授权许可】

Unknown