Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Revista Brasileira de Farmacognosia

Daniela A. Marques²  Mary A. Foglio¹  Patrícia G. Morgante¹  Marie-anne Van Sluys¹  Simone L. Kirszenzaft Shepherd² 

[1] ,Universidade Estadual de Campinas Departamento de Fisiologia Vegetal Campinas SP ,Brasil

关键词: Artemisia annua;    terpene;    in vitro roots culture;    hairy roots;    light;    Artemisia annua;    terpeno;    cultura de raízes in vitro;    raízes transgênicas;    luz;   

DOI  :  10.1590/S0102-695X2006000300003

来源: SciELO

【 摘 要 】

Methodologies were developed for the establishment and cultivation of Artemisia annua L (CPQBA 2/39 x PL5 hybrid) roots submitted to light conditions and genetic transformation performed with Agrobacterium rhizogenes (15834 and 8196 strains). The transgenic and non-transgenic (normal) roots were cultured in Murashige and Skoog (1962) medium, kept under different photoperiodic conditions and analyzed for evaluation of the antiulcerogenic dihydro-epideoxyarteannuin B (compound A) contents. The Dot Blot technique was used to confirm the transgenic nature of the roots. The plants¢s crude extracts were analyzed by Gas Chromatography coupled to Mass Spectrum (CG/MS). The chromatograms of the extracts taken from normal roots revealed the presence of dihydro-epideoxyarteannuin B and other compound (compound B). Photoperiods during cultivation influenced the production of these two compounds: under continuous darkness dihydro-epideoxyarteannuin B was intensely produced and the compound B present in small amounts, while on 16 h photoperiod, the inverse occurred. The quantification of dihydro-epideoxyarteannuin B by Gas Chromatography coupled to Flame Detector Ionization (CG/FID) revealed an approximately fivefold increase in the production of this compound by normal roots kept under continuous darkness compared to roots kept under 16 h light period. The terpene dihydro-epideoxiarteannuin B was not present in transgenic hairy roots.

【 授权许可】

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