Polyploid response of Artemisia annua L. to colchicine treatment

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Yunus, A.^1 ; Parjanto^1 ; Samanhudi^1 ; Hikam, M.P.^2 ; Widyastuti, Y.^3

Lecturer of Study Program of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta, Indonesia^1

Program of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta, Indonesia^2

Research Center for Medicinal Plant and Traditional Drug, Tawangmangu, Indonesia^3

关键词: Artemisia annua;    Chlorophyll contents;    Chromosome numbers;    Completely randomized designs;    Medicinal herb;    Morphological characteristic;    Plasmodium falciparum;    Polyploidization;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012020/pdf
DOI  :  10.1088/1755-1315/142/1/012020

来源: IOP

【 摘 要 】

Artemisia (Artemisia annua) is a a medicinal herb originated from Asia, its contains Artemisinin for malaria (caused by Plasmodium falciparum) treatment. Artemisinin content in A. annua are relatively low, ranging from 0.01% -0.5%. In order to increase the Artemisinin content, polyploid induction could be one effort to be done. For that, this experiment aims to examine the effect of colchicine on morphological characteristics and the induction of polyploidization in Artemisia plants. Polyploid induction on Artemisia annua L. seeds was performed by soaking the Artemisia seeds in colchicine (0%, 0,05%, 0,1% and 0,2%; concentration based) for 2 hours. The experimental design was Completely Randomized Design, one factor, 4 colchicine treatments and in each treatment 7 replicate. The results showed that polyploid occur in plants treated with 0.05% colchicine concentration and its morphological characteristic are 89.4 cm height, 30 branches, 15.9 CCI chlorophyll content, 0.78 cm stem diameter, and chromosome number 2n = 27. In the stomata density of polyploid plants (treated by 0.05% colchicine) was 130 number/mm2with stomata diameter of 22.8 μm.