Application of Metabolic Engineering to the Production of Scopolamine

Molecules

Javier Palazón²  Arturo Navarro-Oca༚¹  Liliana Hernandez-Vazquez² 

[1] Departamento de Alimentos y Biotecnologia, Facultad de Química, Universidad Nacional Autónoma de México, México DF, México; E-mail:;Laboratori de Fisiologia Vegetal, Facultat de Farmacia, Universitat de Barcelona, Avda Diagonal 643, E-08028 Barcelona, Spain; E-mail:

关键词: Scopolamine;    hyoscyamine;    hairy roots;    putrescine N-methyltransferase;    hyoscyamine-6-β-hydroxylase;   

DOI  :  10.3390/molecules13081722

来源: mdpi

【 摘 要 】

Scopolamine is an alkaloid widely used in medicine for its anticholinergic activity. The aim of this review is to show that metabolic engineering techniques constitute a suitable tool to improve the production of tropane alkaloids, focusing in particular on scopolamine. We present an overview of results obtained by various research groups, including our own, who have studied the overexpression of genes involved in the biosynthesis of scopolamine in different plant species that produce tropane alkaloids. Experiments carried out to improve production in hairy root cultures will also be described, as well as those attempting to biotransform hyoscyamine into scopolamine in roots and transgenic tobacco cells.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.