Biotechnology approaches for production of antiulcerogenic dihydro-epideoxyarteannuin B isolated from Artemisia annua L.

Revista Brasileira de Farmacognosia

Van Sluys, Marie-Anne¹  Shepherd, Simone L. Kirszenzaft¹  Universidade de São Paulo, São Paulo, Brasil¹  Marques, Daniela A.¹  Universidade Estadual de Campinas, Paulínia, Brasil¹  Morgante, Patrícia G.¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Registro, Brasil¹  Foglio, Mary A.¹ 

关键词: Artemisia annua;    terpene;    in vitro roots culture;    hairy roots;    light.;   

DOI  :  10.1590/S0102-695X2006000300003

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Methodologies were developed for the establishment and cultivation of Artemisia annua L (CPQBA 2/39 x PL5 hybrid) roots submitted to light conditions and genetic transformation performed with Agrobacterium rhizogenes (15834 and 8196 strains). The transgenic and non-transgenic (normal) roots were cultured in Murashige and Skoog (1962) medium, kept under different photoperiodic conditions and analyzed for evaluation of the antiulcerogenic dihydro-epideoxyarteannuin B (compound A) contents. The Dot Blot technique was used to confirm the transgenic nature of the roots. The plants¢s crude extracts were analyzed by Gas Chromatography coupled to Mass Spectrum (CG/MS). The chromatograms of the extracts taken from normal roots revealed the presence of dihydro-epideoxyarteannuin B and other compound (compound B). Photoperiods during cultivation influenced the production of these two compounds: under continuous darkness dihydro-epideoxyarteannuin B was intensely produced and the compound B present in small amounts, while on 16 h photoperiod, the inverse occurred. The quantification of dihydro-epideoxyarteannuin B by Gas Chromatography coupled to Flame Detector Ionization (CG/FID) revealed an approximately fivefold increase in the production of this compound by normal roots kept under continuous darkness compared to roots kept under 16 h light period. The terpene dihydro-epideoxiarteannuin B was not present in transgenic hairy roots.

【 授权许可】

Unknown