Toxoplasma gondii in experimentally infected Bos taurus and Bos indicus semen and tissues

Pesquisa Veterinária Brasileira

Leslie Scarpelli²  Welber Daniel Zanetti Lopes²  Matheus Migani²  Katia Denise Saraiva Bresciani¹  Alvimar José Da Costa² 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Centro de Pesquisas em Sanidade AnimalJaboticabal SP ,Brazil

关键词: Cattle;    PCR;    semen;    Toxoplasma gondii;    Apicomplexa;    Bovinos;    PCR;    sêmen;    Toxoplasma gondii;    Apicomplexa;   

DOI  :  10.1590/S0100-736X2009000100009

来源: SciELO

【 摘 要 】

Eighteen young steers were inoculated with Toxoplasma gondii and randomly distributed into three groups of six animals each: GI, 2.5x10(5) "P" strain oocysts, GII, 5.0x10(6) "RH" strain tachyzoites, and GIII (Control). Clinical, serological and parasitemia exams were realized. Parasite investigation by bioassay and PCR was realized on semen and fragments of skeletal musculature, lymph nodes, brain, retina, spleen, liver, lung, testicle, epididymis and seminal vesicle. Blood and semen samples were collected on days -2, -1, 1, 3, 5, 7, 14 and weekly thereafter, up to postinfection day (PID) 84. The inoculated steers (GI and GII) presented hyperthermia from PID 3 to 16. Antibodies against T. gondii were detected through the indirect fluorescence antibody test (IFAT) on PID 5 (1:16) in both inoculated groups (oocysts and tachyzoites), reaching peaks of 1:4096 on PID 7. Parasitemia outbursts occurred in all infected bovines, principally from PID 7 to 28, independent of the strain and inoculate used. Bioassays revealed the presence of parasites in semen samples of animals infected with oocysts (GI) and tachyzoites (GII) on several experimental days between PID 7 and 84. Tissue parasitism by T. gondii was diagnosed by bioassay and the PCR technique in several organ and tissue fragments. These findings suggest the possibility of sexual transmission of T. gondii in the bovine species.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License