Cloning and molecular analysis of Toxoplasma gondii Surface Antigen 2 (SAG2) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Monica, W.^1 ; Prasetyo, A.A.^1,2 ; Haryati, S.^1

A-IGIC (A-Infection Genomic Immunology and Cancer), Research Group, Sumber, Banjarsari, Surakarta

57138, Indonesia^1

Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^2

关键词: Bioinformatics analysis;    Blood samples;    Iso-electric points;    Molecular analysis;    Physico-chemical analysis;    Secondary structures;    Surface antigen;    Toxoplasma gondii;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012115/pdf
DOI  :  10.1088/1757-899X/434/1/012115

来源: IOP

【 摘 要 】

The Toxoplasma gondii Surface Antigens 2 (SAG2) is an immunogenic antigen covering the Toxoplasma gondii and one of the main proteins expressed on the surface of the parasite tachyzoites. However, data of the Toxoplasma gondii SAG2 from Indonesian isolate is limited. To study the Toxoplasma gondii SAG2 from Indonesian isolate the gene for SAG2 was amplified from genomic DNA of Toxoplasma gondii isolated from a Javanese acute toxoplasmosis blood samples patient. The clone was sequencing, and the results were subjected to bioinformatics analysis. Physicochemical analysis revealed the 190 aa of SAG2 had 19.47 kDa of weight. The isoelectric point and aliphatic index were 8.61 and 73.105, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. These findings would contribute information about Toxoplasma gondii SAG2 from Indonesian isolate.