Construction and molecular analysis of hepatitis B virus core 1-144 + human immunodeficiency virus Gag recombination from Indonesian isolates

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Prasetyo, A.A.^1,2 ; Permata, A.T.^1 ; Marwoto, M.^1

A-IGIC (A-Infection Genomic Immunology and Cancer), Research Group, Sumber, Banjarsari, Surakarta

57138, Indonesia^1

Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^2

关键词: Bioinformatics analysis;    Epitope predictions;    Human immunodeficiency virus;    Human immunodeficiency virus infections;    Iso-electric points;    Molecular analysis;    Physico-chemical analysis;    Recombinant plasmid;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012113/pdf
DOI  :  10.1088/1757-899X/434/1/012113

来源: IOP

【 摘 要 】

The prevalence of hepatitis B virus and human immunodeficiency virus infection is still high. One strategy to develop diagnostic and/or vaccine for the infection is by construction the recombinant protein from both viruses. To study the characterization of hepatitis B core 1-144 + human immunodeficiency virus Gag recombinant from Indonesian isolate, the hepatitis B virus core 1-144 was amplified from genomic HBV DNA isolated from Javanese blood samples. The human immunodeficiency virus Gag gene was amplified from pVLP HIV Gag, an expression plasmid expressing the human immunodeficiency virus Gag. The clones were then fused and sub cloned into an Escherichia coli expression plasmid. The recombinant plasmid was sequencing, and the recombinant gene sequencing results were subjected to bioinformatics analysis. Physicochemical analysis revealed the molecular weight (Mw), estimated half-life, instability index, isoelectric point, aliphatic index, and hydrophilicity of the recombinant protein. The antigenicity and epitope prediction also had been discussed. The results of this study would contribute information about hepatitis B core 1-144 + human immunodeficiency virus Gag recombinant from Indonesian isolate and benefits for further works willing to develop diagnostic and vaccine against the viruses.