会议论文详细信息
4th International Conference on Energy Equipment Science and Engineering | |
Structure Analysis of a Pathogenesis-Related 10 Protein from Gardenia jasminoides | |
Gao, Lan^1 | |
School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, Guangdong | |
510006, China^1 | |
关键词: Bioinformatics analysis; Hydrophobic cavities; Open reading frame; Pathogenesis-related proteins; Secondary metabolism; Structure analysis; Three-dimensional model; Three-dimensional structure; | |
Others : https://iopscience.iop.org/article/10.1088/1755-1315/242/4/042005/pdf DOI : 10.1088/1755-1315/242/4/042005 |
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来源: IOP | |
【 摘 要 】
The PR-10 proteins belongs to the huge family of pathogenesis related proteins ubiquitous in the plant kingdom, and it includes major food and tree pollen allergens, major latex proteins (MLPs) and cytokinin-specific binding proteins(CSBPs). In this work, a fruit cDNA library of Gardenia jasminoides was constructed, and the GjPR10 cDNA was isolated from the library by sequencing method. The GjPR10 cDNA contains a predicted 486 bp open reading frame, the putative protein contains 161 amino acids, and the protein has a calculated molecular mass 17.7kDa and a theoretical pI 6.25, these characters consistent with usual plant PR10 protein features. A bioinformatics analysis was conducted with previously characterized PR-10 proteins from other plant species. A three-dimensional model of GjPR10 was built. The bioinformatics analysis suggests that the GjPR10 present the conserved P-loop motif. The three-dimensional structure of GjPR10 has the significant common feature of PR10 proteins, it is composed of three alpha-helices and a seven-stranded antiparallel beta-sheet arranged to form a large internal hydrophobic cavity. The results suggest that GjPR10 is a typical PR10 protein, GjPR10 might be involved in plant defence response, developmental processes and in secondary metabolism.【 预 览 】
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Structure Analysis of a Pathogenesis-Related 10 Protein from Gardenia jasminoides | 1348KB | download |