Bioinformatics Analysis of the Genes CYP79B2 in Cabbage (Brassica oleracea var. capitata)

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Sun, Bo^1 ; Jiang, Min^1 ; Yuan, Qiao^1 ; Zhang, Fen^1 ; Tang, Haoru^1

College of Horticulture, Sichuan Agricultural University, Chengdu

611130, China^1

关键词: Amino acid proteins;    Bioinformatics analysis;    Brassica oleracea;    Cytochrome P450 monooxygenases;    Molecular basis;    Open reading frame;    Plant evolution;    Subcellular localizations;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022031/pdf
DOI  :  10.1088/1757-899X/394/2/022031

来源: IOP

【 摘 要 】

CYP79B2 is an important cytochrome P450 monooxygenases in glucosinolate biosynthesis. Here, the Brassica oleracea var. capitata CYP79B2 (BocCYP79B2) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocCYP79B2.1, BocCYP79B2.2 and BocCYP79B2.3 genes mapped to chromosomes 1, 3 and 7, and contains an open reading frame of 1,623 bp, 1,557 bp and 1,626 bp that encodes a 540, 518, 541 amino acid protein, respectively. Subcellular localization predicted all BocCYP79B2 genes were in the chloroplast. The conserved domain of the BocCYP79B2 protein is PLN02971. Homology analysis indicates that the CYP79B2 protein is apparently conserved during plant evolution. The findings of the present study provide a molecular basis for the elucidation of CYP79B2 gene function in cabbage.