Insecticidal activities of crude extact of Metarhizium anisopliae and conida suspension against Crocidolomia pavonana fabricius

Humanosphere Science School 2017; 7th International Symposium for a Sustainable Humanosphere

地球科学;经济学

Melanie^1 ; Miranti, M.^2 ; Kasmara, H.^2 ; Hazar, S.^3 ; Martina, A.^3

Student of Environtmental Science Doctoral Programme, Faculity of Post-Graduated School, Padjadjaran University, Jl. Dipatiukur No. 35, Jawa Barat, Bandung

45363, Indonesia^1

Departement of Biology, Faculty Mathematics and Natural Sciences, Padjadjaran University, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, Jawa Barat

45363, Indonesia^2

Biology Departement, Faculty Mathematics and Natural Sciences, Padjadjaran University, Jawa Barat

45363, Indonesia^3

关键词: Brassica oleracea;    Completely randomized designs;    Concentration levels;    Insect population;    Insecticidal activity;    Metarhizium anisopliae;    Plutella xylostella;    Spodoptera litura;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/166/1/012017/pdf
DOI  :  10.1088/1755-1315/166/1/012017

来源: IOP

【 摘 要 】

Pest resistance, resurgent and the residual effect of conventional insecticides are the basis to apply Insect Pest Management (IPM) strategies. Metarhizium anisopliae is an entomophatogenic fungus that has considerable promise for usage in IPM. The spores of fungus had great potential as insect pathogen, in addition the mycotoxins products having current interest as bioactive agent. M. anisopliae was exhibited to control pest insect populations of crops especially host plant of Brassica oleracea (Cruciferae), i.e. Plutella xylostella, Spodoptera litura, and Crocidolomia pavonana. The insecticidal activities of conidia suspensions and crude extract product containing mycotoxins were investigated, withthe parametersmortality and lethal time of C. pavonana 3rd instar larvae. The experimental design of crude extract and conidia suspensions of M. anisopliae on mortality effect used Completely Randomized Design (RAL). The bioassay of conidia suspensions mortality test used 6 level spore concentrations (0, 105, 106, 107, 108, and 109 spores/ml). Analysis of variance (ANAVA) at α 95% followed by Duncan multiple test at α 5% was carried out. The result of crude extract mortality test showed that M. anisopliae produced mycotoxins from the third day of fungal growth. The long growth of fungus is directly proportional with the produced mycotoxins. The mycotoxins was optimally produced in 7th days. The extract M. anisopliae at highest mortality effect on larvae was tested for 24 to 120 h. One hundred percent mortality of larvae were achieved at 120 h with an average lethal time were 3.6 days shorter than mortality caused by conidia infections. Thus, conidia infections showed that M. anisopliae at 105 - 109 spores/ml concentrations led to mortalities of 75%, 90%, 80%, 85%, and 95 %, respectively (P9 spores/ml concentration.