Application of UPLC-MS/MS for Simultaneous Determination of Malachite Green and Metabolites Residues in Tilapia

2019 International Conference on Advanced Electronic Materials, Computers and Materials Engineering

无线电电子学;计算机科学;材料科学

Zeng, Shaodong^1^2 ; Ye, Jianzhi^1^2 ; Lin, Ling^1^2 ; Chen, Wuhai^1^2 ; Yang, Chunliang^1^2

Agriculture Products Processing Research Institute, Chinese Academy of Tropical Agriculture Sciences, Zhanjiang,Guangdong

524001, China^1

Laboratory of Quality and Safety Risk Assessment on Agro-products Processing (Zhanjiang), Ministry of Agriculture and Rural Affairs of PRC, Zhanjiang,Guangdong

524001, China^2

关键词: Accuracy and precision;    Concentration levels;    Leucomalachite green;    Relative standard deviations;    Sensitive method;    Simultaneous determinations;    Simultaneous monitoring;    Ultra-high performance liquid chromatographies;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/563/5/052062/pdf
DOI  :  10.1088/1757-899X/563/5/052062

来源: IOP

【 摘 要 】

In this work, a convenient, sensitive method for simultaneous monitoring of malachite green, leucomalachite green in tilapia samples was developed by ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The tilapia samples were extracted by acetonitrile, separated withacetonitrile-5mmol/L ammonium acetate as mobile phase, and then detected under ESI+ mode. The method showed a good linearity for all the analysts over the range of 0.1-100 μg kg-1 with r2≥0.998. And the detection limits both were 0.10 μg kg-1 for Malachite green and leucomalachite green. Tests for recovery were made by addition of standards at four different concentration levels (0.5, 2.0, 10.0, 20.0 μg kg-1) to the blank sample. The recoveries of the four spiked concentrations were ranged from 81.5% to 104%, with the relative standard deviations lower than 5%. The sensitivity, accuracy and precision of this method were able to meet the requirements for malachite green and leucomalachite green residue analysis.