Superdioxide dismutase (SOD) and metallothionein (MT) Tubifex tubifex at the acute mercury exposure

1st International Conference on Fisheries and Marine Science

Widiastuti, I.M.^1 ; Hertika, A.M.S.^2 ; Musa, M.^3 ; Arfiati, D.^3

Doctoral Program, Faculty of Fisheries and Marine, Science Universitas Brawijaya, Malang, Indonesia^1

Aquaculture Study Program, Faculty of Animal Husbandry and Fisheries, Tadulako University, Palu, Indonesia^2

Faculty of Fisheries and Marine, Science Brawijaya University, Malang, Indonesia^3

关键词: Acute toxicity test;    Concentration levels;    Mercury exposure;    Metallothioneins;    Pathological conditions;    Super oxide dismutase;    Superoxide dismutases;    Tubifex tubifex;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/236/1/012101/pdf
DOI  :  10.1088/1755-1315/236/1/012101

来源: IOP

【 摘 要 】

Oxidative stress is a condition of over production of free radicals that can cause cell damage, pathological conditions and cell death. This study aims was to determined the activity of superoxide dismutase (SOD) and metallothionein (MT) levels in Tubifex tubifex worms exposed to HgCl2 in various concentration levels. The concentration of HgCl2 used was based on LC50-48 h values in acute toxicity test (15,85 μg / L, 25,12 μg / L; 38,81 μg / L; 63,09 μg / L; 99,98 μg / L ). The method used was experiment. Determination of SOD activity done using spectrophotometry and MT measurement using ELISA method. The results was showed that HgCl2 exposure to Tubifex tubifex caused increased levels of superoxide dismutase enzyme and metallothionein levels. SOD and MT levels were directly proportional to the concentration of HgCl2, the higher the concentration, SOD and MT levels will increase accordingly.