Methallothionein expression on the gills and stomach of Chinese pond mussels exposed to lead (Pb)

Asean-Fen International Fisheries Symposium - 2017

Kartikaningsih, H.^1 ; Suryanto, A.M.^1 ; Arfiati, D.^1

Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia^1

关键词: Cell rupture;    Exposed to;    Fresh Water;    Haemorrhage;    Hematoxylin eosin;    Ion accumulations;    Metallothioneins;    Water circulation system;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012058/pdf
DOI  :  10.1088/1755-1315/137/1/012058

来源: IOP

【 摘 要 】

In freshwaters area, Pb originates from rocks (naturally), industries, and pesticides. The ability of Chinese pond mussels as biofilters to absorb heavy metal (Pb) was demonstrated in water circulation system using ten 8 cm mussels. PbNO3(0, 10, 20, and 30 ppm) was administered into water containing mussels. Carp culture was done for 30 days, and Pb accumulation in carps was measured every week (week 0, 1, 2, and 3). The results showed that the highest Pb ion accumulation was found in the gills of mussels. The examination using hematoxylin-eosin showed that tissues were damage due to haemorrhage, cell ruptures, and cell deaths. The results of the measurement of metallothionein (MT) showed that MT molecular weight was 12.84 kDa.