Genome-wide identification of Tomato Golden 2-Like transcription factors and abiotic stress related members screening

BMC Plant Biology

Jing-fu Li¹  Ting-ting Zhao¹  Tong Pei¹  Qi-feng Liu¹  Jun-fang Liu¹  He Zhang¹  Huan-huan Yang¹  Zi-yu Wang¹  Tai-ru Wu¹  Xu-ying Sun¹  Shuang Zhao¹  Yue Wang¹  Xiang-yang Xu¹  Hai-yan Zhao¹  Jing-bin Jiang¹ 

[1] Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture and Landscape Architecture, Northeast Agricultural University, No. 600, Changjiang Road, 150030, Harbin, Heilongjiang Province, P.R. China;

关键词: Tomato;    G2-like;    Bioinformatics analysis;    Expression patterns;    Abiotic stresses;   

DOI  :  10.1186/s12870-022-03460-9

来源: Springer

【 摘 要 】

BackgroundGolden 2-Like (G2-like) transcription factors play an important role in plant development. However, the roles of these G2-like regulatory genes in response to abiotic stresses in tomato are not well understood.ResultsIn this study, we identified 66 putative G2-like genes in tomato (Solanum lycopersicum) and classified them into 5 groups (I to V) according to gene structure, motif composition and phylogenetic analysis. The G2-like genes were unevenly distributed across all 12 chromosomes. There were nine pairs of duplicated gene segments and four tandem duplicated SlGlk genes. Analysis of the cis-regulatory elements (CREs) showed that the promoter regions of SlGlks contain many kinds of stress- and hormone-related CREs. Based on RNA-seq, SlGlks were expressed in response to three abiotic stresses. Thirty-six differentially expressed SlGlks were identified; these genes have multiple functions according to Gene Ontology (GO) analysis and are enriched mainly in the zeatin biosynthesis pathway. Further studies exhibited that silencing SlGlk16 in tomato would reduce drought stress tolerance by earlier wilted, lower superoxide dismutase (SOD), peroxidase (POD) activities, less Pro contents and more MDA contents.ConclusionsOverall, the results of this study provide comprehensive information on G2-like transcription factors and G2-like genes that may be expressed in response to abiotic stresses.

【 授权许可】

CC BY