Quantification of tryptophan in plasma by high performance liquid chromatography

Química Nova

Renata Romanholi Pinhati²  Hudson Caetano Polonini²  Marcos Antônio Fernandes Brandão²  Nádia Rezende Barbosa Raposo²  Felipe Corchs¹  Wagner Farid Gattaz¹ 

[1] ,Universidade Federal de Juiz de Fora Faculdade de Farmácia Juiz de Fora MG ,Brasil

关键词: tryptophan;    serotonin;    HPLC;   

DOI  :  10.1590/S0100-40422012000300032

来源: SciELO

【 摘 要 】

A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.

【 授权许可】

CC BY-NC   
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