Determinação simultânea de precursores de serotonina - triptofano e 5-hidroxitriptofano - em café

Química Nova

Ana Carolina C. L. Martins¹  Tarliane M. Silva¹  M. Beatriz A. Gloria¹ 

[1] ,Universidade Federal de Minas Gerais Faculdade de Farmácia Departamento de AlimentosBelo Horizonte MG ,Brasil

关键词: coffee;    serotonin;    tryptophan;   

DOI  :  10.1590/S0100-40422010000200016

来源: SciELO

【 摘 要 】

Epidemiological studies attributed positive effects in the central nervous system (CNS) to coffee. Among possible active constituents, serotonin, a neurotransmitter in the CNS, is present; but dietary sources do not cross the blood-brain barrier. Tryptophan and 5-hidroxytryptophan (5-HTP) are serotonin precursors and can affect brain concentrations. An ion-pair-HPLC, post-column derivatization with o-phthalaldehyde and fluorimetric detection before and after hydrolysis with NaOH and extraction with methanol:water was developed for the simultaneous determination of these compounds. It was selective, sensitive (LOD = 0.3 and 0.2 μg/mL), precise (91.3 and 94.2% recovery for tryptophan and 5-HTP, respectively), and linear from 0.3 to 40 μg/mL for both compounds. It was applied to green and roasted arabica and robusta coffees.

【 授权许可】

CC BY-NC   
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