A holistic approach was applied in the determination of Trp:Lys ratio in 10 to 20 kg pigs. The objective of the first experiment was to determine the standardized ileal digestible (SID) AA of specific allocations of ingredients identified to be low in Trp:Lys ratio. Results indicated that the SID of AA in CGM was similar to the SID of AA in SBM. The SID of AA in field peas was similar to that of corn, however each were somewhat lower (P < 0.05) than the SID of AA in SBM and CGM. Possibly because of overheating, the SID of AA in HP DDG was lower (P < 0.05) than the other ingredients, especially in Lys. The objective of the second set of experiments was to obtain Lys and Trp requirements of 10 to 20 kg pigs fed either a corn-CGM-field peas or a corn-HP DDG diet to aid in the development of the basal diet for subsequent experiments. Three experiments were conducted, first, determining the Lys requirement in a corn-SBM diet, second, comparing the growth performance of pigs fed various diet types, and third, determination of Lys and Trp requirement in corn-CGM-field pea diets. In Exp. 1, SID Lys requirement estimates of 1.18 and 1.29%, respectively were obtained. In Exp. 2, five dietary treatments were developed to contain a corn-SBM diet, a corn-CGM-field peas diet with 2 levels of SID Lys (1.20 and 1.35%), and a corn-HPDDG diet 2 levels of SID Lys (1.20 and 1.35%). The pigs fed the corn-SBM diet resulted in higher (P < 0.05) growth performance than pigs fed any of the corn-CGM-field pea or the corn-HP DDG diets. Pigs fed the corn-CGM-field pea diet and corn-HP DDG diet formulated to 1.35% SID Lys had greater (P < 0.05) growth performance than pigs fed similar diets formulated to 1.20%. In Exp. 3, Trp and Lys requirements were determined to be 0.21% and 1.21%, respectively, using the average of the intercepts of broken line and quadratic analyses of ADG, G:F, and PUN. The objective of the third set of experiments was to confirm the formulated basal to be used in the determination of Trp:Lys ratio was limiting Lys and Trp. Using PUN as the response criteria, the basal diets were found to be limiting in both Lys and Trp. The objective of the fourth set of experiments was to determine the SID Trp:Lys ratio in 10 to 20 kg pigs using a corn-CGM field pea diet (Exp. 1) or a corn-HP DDG diet (Exp. 2). Diets were formulated to contain increasing levels of SID Trp:Lys from 11.7 to 23.5% using 6 diets. The Trp:Lys ratio was determined to be 18.2% using the average of the broken line analyses for both basal diets.
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Estimation of the ideal standardized ileal digestible Tryptophan:Lysine ratio in 10 to 20 kg pigs