A simple and economic slide micro-immunoenzymatic (Micro-SIA) test for epidemiological studies of toxoplasmosis

Memórias do Instituto Oswaldo Cruz

Mara Rosenzvit²  Sergio Angel¹  Luis Alvarez¹  Viviana Mellare¹  Jorge Blanco¹  Viviana Pszenny¹  Juan Carlos Garberi¹ 

[1] ,Instituto de Investigaciones Médicas "Alfredo Lanari" Departamentos de Sustancias Vasoactivas Buenos Aires,Argentina

关键词: toxoplasmosis;    serodiagnosis;    microELISA;    epidemiology;   

DOI  :  10.1590/S0074-02761994000100009

来源: SciELO

【 摘 要 】

A slide micro-immunoenzymatic assay (micro-SIA) to detectantibodies to non-particulate Toxoplasma gondii antigens is described. This assay allows the diagnosis of toxoplasmosis infection in about 1 hr. Twenty-four determinations can be performed per slide. Five hundred ng of antigen and 5 or 10 µl drop of each reactive are necessary per well. The clear contrast of colours obtained for negative and positive sera after the test is finished, allows direct discrimination of the results. However, it is possible to quantify the results of the reaction using a minireader. Sera dilution cutoff value, determined as themost frequent titre for the general population, is 1:100. The toxoplasma micro-SIA correlates well with indirect immunofluorescence (IIF), its sensitivity is atleast three times as much as IIF. The test has an intra and inter assay variation coefficient of 5.46 per cent and of 6.24 per cent respectively. Sera obtained at random from argentinian people were analyzed and a 56 per cent of infection was found. The main features of the Toxoplasma micro-SIA are its simplicity, sensitivity, reproducibility, and the virtual absence of background making it very suitable for screening tests.

【 授权许可】

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