Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

The Journal of Veterinary Medical Science

article

Yang GAO¹  Takehisa SOMA²  Xuenan XUAN¹  Huanping GUO¹  Paul Franck Adjou MOUMOUNI¹  Mingming LIU¹  Jixu LI¹  Artemis EFSTRATIOU¹  Eloiza May GALON¹  Rochelle Haidee YBANEZ¹  Tatsunori MASATANI³ 

[1] National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine;Veterinary Diagnostic Laboratory, Ltd.;Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University

关键词: cat;    enzyme-linked immunosorbent assay;    serodiagnosis;    SAG1-related sequence 2 of Toxoplasma gondii;    toxoplasmosis;   

DOI  :  10.1292/jvms.20-0231

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

An enzyme-linked immunosorbent assay (ELISA) based on recombinant SAG1-related sequence 2 of Toxoplasma gondii (rTgSRS2) was developed to detect toxoplasmosis in cats. The specificity and sensitivity of rTgSRS2 ELISA were confirmed using a series of serum samples from T. gondii -experimentally infected mice. A total of 76 field samples from cats were examined by the developed ELISA. The rTgSRS2 ELISA showed a good diagnostic performance characterized by high concordance (88.16) and kappa value (0.76) with latex agglutination test (LAT). The sensitivity and specificity of the test were 92.68% and 82.86%, respectively. These results suggest that the ELISA based on rTgSRS2 could be a useful tool for serodiagnosis of T. gondii infection in cats.

【 授权许可】

Unknown