Radiometric studies on the oxidation of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria

Revista do Instituto de Medicina Tropical de São Paulo

Edwaldo E. Camargo¹  Teresa M. Kopajtic¹  Glinda K. Hopkins¹  Nancy P. Cannon¹  Henry N. Wagner Jr¹ 

[1] ,The Johns Hopkins Medical InstitutionsBaltimore Maryland

关键词: Radiometric assay;    M. tuberculosis;    (U-14C) L-Amino Acids;    Drug resistance;   

DOI  :  10.1590/S0036-46651987000500008

来源: SciELO

【 摘 要 】

A radiometric assay system has been used to study oxidation patterns of (U-14C) L-amino acids by drug-susceptible and drug-resistant mycobacteria. Drug-susceptible M. tuberculosis (H37Rv TMC 102 and Erdman) along with the drug-resistant organism M. tuberculosis (H37 Rv TMC 303), M. bovis, M. avium, M. intracellulare, M. kansasii and M. chelonei were used. The organisms were inoculated into a sterile reaction system with liquid 7H9 medium and one of the (U-14C) L-amino acids. Each organism displayed a different pattern of amino acid oxidation, but these patterns were not distinctive enough for identification of the organism. Complex amino acids such as proline, phenylalanine and tyrosine were of no use in identification of mycobacteria, since virtually all organisms failed to oxidize them. There was no combination of substrates able to separate susceptible from resistant organisms.

【 授权许可】

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