Cytokines for evaluation of chronic inflammatory status in ageing research: reliability and phenotypic characterisation

Immunity & Ageing

Andreas F. H. Pfeiffer¹  Olga Pivovarova-Ramich²  Tilman Grune³  Liselot Koelman⁴  Krasimira Aleksandrova⁴ 

[1] 0000 0004 0390 0098, grid.418213.d, Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany;grid.452622.5, German Center for Diabetes Research (DZD), Neuherberg, Germany;0000 0001 2218 4662, grid.6363.0, Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany;0000 0004 0390 0098, grid.418213.d, Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany;grid.452622.5, German Center for Diabetes Research (DZD), Neuherberg, Germany;0000 0001 2218 4662, grid.6363.0, Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany;0000 0004 0390 0098, grid.418213.d, Senior Scientist Group Molecular Nutritional Medicine, Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany;0000 0004 0390 0098, grid.418213.d, Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany;0000 0004 0390 0098, grid.418213.d, Senior Scientist Group Nutrition, Immunity and Metabolism, Department of Nutrition and Gerontology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany;University of Potsdam, Institute of Nutritional Science, Potsdam, Germany;

关键词: Reliability;    Cytokines;    Multiplex platforms;    Inflammaging;    Biomarkers;    Ageing;    BMI;   

DOI  :  10.1186/s12979-019-0151-1

来源: publisher

【 摘 要 】

BackgroundThere is a growing interest in the role of inflammageing for chronic disease development. Cytokines are potent soluble immune mediators that can be used as target biomarkers of inflammageing; however, their measurement in human samples has been challenging. This study aimed to assess the reliability of a pro- and anti-inflammatory cytokine panel in a sample of healthy people measured with a novel electrochemiluminescent multiplex immunoassay platform (Meso Scale Discovery, MSD), and to characterize their associations with metabolic and inflammatory phenotypes.ResultsOverall, the majority of cytokines were above the limit of detection (in at least 85.3% of the samples). Cytokines IL-6, IL-8, TNF-α, IL-10, IL-13, and IFN-γ showed overall good to fair reliability (ICC > 0.40), whereas IL-1β, IL-2, IL-4, and IL-12p70 showed poor reliability (ICC < 0.40). The reliability estimates were not substantially influenced by participants’ age, sex, obesity and C-reactive protein (CRP) levels. As expected, cytokine concentrations were elevated with advanced age most pronouncedly for IL-6, IL-8, Il-2, IFN- γ, and TNF-α. No major associations with metabolic phenotypes were observed for most cytokines, with the exception of a positive association between IL-6 and TNF-α with body mass index and CRP (ρ: 0.36; ρ: 0.20; ρ: 0.53; ρ: 0.22, respectively), and IFN-γ and IL-10 with CRP (ρ: 0.23 and ρ: 0.19, respectively).ConclusionsSingle measurements of selected cytokines using MSD platform, including IL-6, IL-8, IL-10, IL-13, TNF-α, and IFN-γ have shown to be representative of an individual’s average level over time and could be suitable for use in prospective epidemiological and clinical studies. Such studies are highly warranted to characterize associations of cytokines with phenotypes and diseases associated with ageing.

【 授权许可】

CC BY