Extracts from Cladiella australis, Clavularia viridis and Klyxum simplex (Soft Corals) are Capable of Inhibiting the Growth of Human Oral Squamous Cell Carcinoma Cells

Marine Drugs

Chia-Hua Liang⁴  Guey-Horng Wang⁴  Chih-Chuang Liaw²  Mei-Feng Lee³  Shih-Hao Wang²  Da-Long Cheng¹ 

[1] Department of Computer and Communication, Shu-Te University, Kaohsiung County, 82445 Taiwan;Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung, 404, Taiwan;Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan;Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, 717, Taiwan

关键词: Soft corals;    Cladiella australis;    Clavularia viridis;    Klyxum simplex;    Apoptosis;   

DOI  :  10.3390/md6040595

来源: mdpi

【 摘 要 】

Many biomedical products have already been obtained from marine organisms. In order to search more therapeutic drugs against cancer, this study demonstrates the cytotoxicity effects of Cladiella australis, Clavularia viridis and Klyxum simplex extracts on human oral squamous cell carcinoma (SCC4, SCC9 and SCC25) cells using cell adhesion and cell viability assay. The morphological alterations in SCCs cells after treatment with three extracts, such as typical nuclear condensation, nuclear fragmentation and apoptotic bodies of cells were demonstrated by Hoechst stain. Flow cytometry indicated that three extracts sensitized SCC25 cells in the G₀/G₁ and S-G₂/M phases with a concomitant significantly increased sub-G₁ fraction, indicating cell death by apoptosis. This apoptosis process was accompanied by activation of caspase-3 expression after SCC25 cells were treated with three extracts. Thereby, it is possible that extracts of C. australis, C. viridis and K. simplex cause apoptosis of SCCs and warrant further research investigating the possible anti-oral cancer compounds in these soft corals.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.