The Ethanolic Extracts The Gorgonian Isis hippuris Inhibited the Induced Mammary Carcinoma Growth In C3H Mice

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Trianto, Agus^1,4 ; Andriyas, Yogi^1 ; Ridlo, Ali^1 ; Sedjati, Sri^1 ; Susilaningsih, Neni^2 ; Murwani, Retno^3,4

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^1

Department of Histology, Faculty of Medicine Diponegoro University, Indonesia^2

Faculty of Animal and AgricultureScience, Laboratory of Physiology Dan Biochemistry, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^3

Natural Product Laboratory, Centre for Research and Services, Diponegoro University, Jl. Prof. Soedharto, SH, Tembalang, Semarang, Indonesia^4

关键词: bioactive;    Ethanolic extracts;    Histological examination;    Kruskal-Wallis tests;    mammae;    Marine biota;    Secondary metabolites;    Soft corals;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012104/pdf
DOI  :  10.1088/1755-1315/116/1/012104

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The gorgonian Isis hippuris contains secondary metabolites gorgosterol and hippuristanol which are capable of inhibiting cancer cells. However, in vivo test of the gorgonian Isis hippuris extract as the anticancer drug has not been conducted. The research to study of the effect of ethanolic extract of the gorgonian on the induced tumor growth in C3H mice. The I. hippuris was obtained from Karimunjawa water in Jepara. The extract was prepared by maceration using ethanol. A total 20, 8-10 moths old of C3H mice with an initial weight of 20-25 gram were assigned into control, Ih-1, Ih-2, and Ih-3 groups. Control, Ih-1, Ih-2, and Ih-3 groups each received 0, 0.15, 1.5, and 15 mg extract per mouse per day respectively for two weeks. Cancer cells were introduced to all groups from a donor cancer mouse by injection via left or right axilla and allowed to grow. The cancer mass was removed and processed for histological examination, and cancer growth was determined according to Elston and Ellis criteria. The result showed that histological grade of cancer mass from the control group was in grade 2 or differentiated moderately. The histological grade of cancer mass from Ih-1, Ih-2, and Ih-3 groups were in grade 1 (low grade) or similar to a normal cell. Statistical analysis by Kruskal-Wallis test showed a significant difference (p