Proliferative Activity of Mammary Carcinoma Cells by AgNOR Count in C3H mice Receiving Ethanol Extract of Sponge Haliclona sp

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Sijabat, Lanceria^1 ; Susilaningsih, Neni^1 ; Trianto, Agus^2,4 ; Murwani, Retno^3,4

Department of Histology, Faculty of Medicine Diponegoro University, Indonesia^1

Laboratory of Marine Biotechnology, Faculty of Fisheries and Marine Science, Diponegoro University, Indonesia^2

Faculty of Animal and Agriculture Science, Laboratory of Physiology Dan Biochemistry, Diponegoro University, Indonesia^3

Natural Product Laboratory, Centre of Research and Services, Diponegoro University, Indonesia^4

关键词: Bioactive compounds;    Breast cancer cells;    Breast tumor;    mammae;    Mammary carcinoma cell;    marine;    Proliferative activity;    Soft corals;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012100/pdf
DOI  :  10.1088/1755-1315/116/1/012100

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Quantification of argyrophilic nucleolar organizer region (AgNORs) was considered as one of markers of proliferative activity of cancer cells. Sponge Haliclona sp extract contains anticancer bioactive compounds and our previous study showed that the extract was able to improve histological grade of induced mammary adenocarcinoma in mice. The following research was conducted to study the extract administration on the proliferative activity of the carcinoma cells represented by AgNOR count in mice. This experimental study applied post test only control group design. Twenty C3H mice were divided into four groups namely C (control), H1, H2and H3. Each group was given 0, 0.15, 1.5, and 15 mg Haliclona sp extract respectively. After three weeks of extract administration, mice were inoculated with breast cancer cells from donor mice. The extract administration were continued for another three weeks. AgNOR count was performed on tumor sections and expressed as mean of AgNOR (mAgNOR) and percentage of AgNOR (pAgNOR). Means of mAgNOR in C, H1, H2and H3were 4.070, 3.195, 3.450, and 3.190 respectively. Means of pAgNOR in C, H1, H2and H3were 34,40, 25,40, 38,40 and 19,80 respectively. The lowest means of mAgNOR and pAgNOR which is an indication of lower proliferative activity of the cancer cells was found in H3. However no significant difference can be found among treatment groups (p>0.05). Using AgNOR count, the ethanol extract of Haliclona sp could not show significant reduction in proliferation of mammary carcinoma cells of C3H mice. This finding support the view that AgNOR alone could not be used to determine pathology of cancer cells.