Teucrium Plant Species as Natural Sources of Novel Anticancer Compounds: Antiproliferative, Proapoptotic and Antioxidant Properties

International Journal of Molecular Sciences

Milan S. Stankovic²  Milena G. Curcic²  Jovana B. Zizic²  Marina D. Topuzovic²  Slavica R. Solujic¹ 

[1] Department of Chemistry, Faculty of Science, University of Kragujevac, Str. Radoja Domanovića No. 12, 34000 Kragujevac, Republic of Serbia; E-Mail:;Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Str. Radoja Domanovića No. 12, 34000 Kragujevac, Republic of Serbia; E-Mails:

关键词: acridin orange/ethidium bromide assay;    antiprolifertative activity;    antioxidants;    MTT assay;    phenols;    Teucrium;   

DOI  :  10.3390/ijms12074190

来源: mdpi

【 摘 要 】

This study deals with total phenolic content, antiproliferative and proapoptotic activity of methanolic extracts from different Teucrium species and the effect on the prooxidant/antioxidant status in HCT-116 cells. The total phenolic content of the extracts was measured spectrophotometricaly and the obtained results ranged from 56.62 mg/g to 172.50 mg GA/g. The antiproliferative activity of methanolic extracts from different Teucrium species was determined using MTT cell viability assay, where IC₅₀ value was used as a parameter for cytotoxicity. The type of cell death was explored by fluorescence microscopy using the acridin orange/ethidium bromide method. MTT assay showed that all extracts significantly reduced cell viability in a dose-dependent manner, with very low IC₅₀ values. The highest content of phenolic compounds and the best cytotoxic activity on HCT-116 cells after 24 h of exposure was in T. chamaedrys extract, with IC₅₀ values of 5.48 × 10⁻⁹ μg/mL. After 72 h, methanolic extract of T. arduini appeared to have the best cytotoxic activity on HCT-116, with IC₅₀ values of 0.37 μg/mL. Treatments caused typical apoptotic morphological changes in HCT-116 cells and showed a high percentage of apoptotic cells. The results of the presented research indicate that some Teucrium extracts are a very rich source of phenols, which may directly contribute to high antiproliferative and proapoptotic activity.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.