Five Withanolides from the Leaves of Datura metel L. and Their Inhibitory Effects on Nitric Oxide Production

Molecules

Bing-You Yang¹  Rui Guo¹  Ting Li²  Yan Liu²  Chang-Fu Wang²  Zun-Peng Shu²  Zhi-Bin Wang²  Jing Zhang²  Yong-Gang Xia²  Hai Jiang²  Qiu-Hong Wang¹ 

[1] id="af1-molecules-19-04548">Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, Chi

关键词: Datura metel L.;    withanolides;    dmetelins;    NO inhibition;   

DOI  :  10.3390/molecules19044548

来源: mdpi

【 摘 要 】

Four new withanolides named dmetelins A–D (compounds 1–4), along with the known compound 7α,27-dihydroxy-1-oxo-witha-2,5,24-trienolide (5) were isolated from the leaves of Datura metel L. (Solanaceae). Their structures were elucidated on the basis of detailed analysis of 1D and 2D NMR and mass spectrometry data. All the compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells. Compounds 1, 4 and 5 showed significant inhibitory activities, and compounds 2 and 3 showed moderate inhibitory activities with IC₅₀ values of 17.8, 11.6, 14.9, 33.3 and 28.6 μM, respectively.

【 授权许可】

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.