Anti-Ageing Effects of Sonchus oleraceus L. (pūhā) Leaf Extracts on H2O2-Induced Cell Senescence

Molecules

Zong-Quan Ou¹  Thomas Rades²  Arlene McDowell¹ 

[1] School of Pharmacy, University of Otago, Dunedin 9056, New Zealand; E-Mail:;Department of Pharmacy, University of Copenhagen, Copenhagen 2100, Denmark; E-Mail:

关键词: Sonchus oleraceus;    antioxidants;    leaf extracts;    caffeic acid derivatives;    CAA;    senescence;    anti-ageing;   

DOI  :  10.3390/molecules20034548

来源: mdpi

【 摘 要 】

Antioxidants protect against damage from free radicals and are believed to slow the ageing process. Previously, we have reported the high antioxidant activity of 70% methanolic Sonchus oleraceus L. (Asteraceae) leaf extracts. We hypothesize that S. oleraceus extracts protect cells against H₂O₂-induced senescence by mediating oxidative stress. Premature senescence of young WI-38 cells was induced by application of H₂O₂. Cells were treated with S. oleraceus extracts before or after H₂O₂ stress. The senescence- associated β-galactosidase (SA-β-gal) activity was used to indicate cell senescence. S. oleraceus extracts showed higher cellular antioxidant activity than chlorogenic acid in WI-38 cells. S. oleraceus extracts suppressed H₂O₂ stress-induced premature senescence in a concentration-dependent manner. At 5 and 20 mg/mL, S. oleraceus extracts showed better or equivalent effects of reducing stress-induced premature senescence than the corresponding ascorbic acid treatments. These findings indicate the potential of S. oleraceus extracts to be formulated as an anti-ageing agent.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.