Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice

International Journal of Molecular Sciences

Ting Min¹  Jie Sun¹  Yang Yi¹  Hong-Xun Wang¹  Fei Hang²  You-Wei Ai¹  Li-Mei Wang¹ 

[1] College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, China; E-Mails:;Sericultural & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture, Guangzhou 510610, China; E-Mail:

关键词: longan pulp;    polysaccharide-protein complex;    HPSEC-FD;    pharmacokinetics;    distribution;   

DOI  :  10.3390/ijms161024403

来源: mdpi

【 摘 要 】

A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide–protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1–200 µg/mL in mouse plasma, spleen and lung samples with correlation coefficients greater than 0.995. The inter-day and intra-day precisions of the method were not more than 6.9%, and the relative recovery ranged from 93.7% to 106.4%. The concentration–time curve of LPPF in plasma following intravenous (i.v.) administration at 40 mg/kg body weight well fitted to a two-compartment model. LPPF rapidly eliminated from plasma according to the short half-lives (t_1/2α = 2.23 min, t_1/2β = 39.11 min) and mean retention times (MRT_0–t = 1.15 h, MRT_0–∞ = 1.39 h). After administration over 5 to 360 min, the concentration of LPPF in spleen homogenate decreased from 7.41 to 3.68 µg/mL; the concentration in lung homogenate decreased from 9.08 to 3.40 µg/mL. On the other hand, the increasing concentration of LPPF fraction with low molecular weight in heart homogenate was observed.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.