Physicochemical Characteristics and Immunomodulatory Activities of Three Polysaccharide-Protein Complexes of Longan Pulp

Molecules

Yang Yi¹  Sen Tai Liao¹  Ming Wei Zhang¹  John Shi¹  Rui Fen Zhang¹  Yuan Yuan Deng¹ 

[1] ¹Ministry of Agriculture/Bio-tech Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China ²College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China ³Guleph Food Research Center, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, ON N1G5C9, Canada

关键词: longan;    pulp;    polysaccharide-protein complex;    physicochemical characteristic;    immunomodulatory activity;   

DOI  :  10.3390/molecules16076148

来源: mdpi

【 摘 要 】

Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.