A Comparative Identification of Ochratoxin A in Longan Fruit Pulp by High Performance Liquid Chromatography-Fluorescence Detection and Electron Spray Ionization-Mass Spectrometry

Molecules

Jing Li¹  Haihui Xie¹  Bao Yang¹  Xinhong Dong¹  Linyan Feng¹  Feng Chen¹ 

[1] ¹South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China ²Department of Food Science and Human Nutrition, Clemson University, Clemson, SC 29634, USA

关键词: ochratoxin A;    longan;    fruit;    pulp;    solid-phase extraction;    HPLC-FD;    ESI-MS;   

DOI  :  10.3390/molecules15020680

来源: mdpi

【 摘 要 】

Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.