Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp

Molecules

Yang Yi¹  Fei Huang¹  Ming-Wei Zhang¹  Rui-Fen Zhang¹  Yuan-Yuan Deng¹  Zhen-Cheng Wei¹ 

[1] Key Laboratory of Functional Food, Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China

关键词: longan pulp;    polysaccharide;    solution property;    conformation;    anti-tumor activity;   

DOI  :  10.3390/molecules180911601

来源: mdpi

【 摘 要 】

The solution properties of four fractions (LPI–IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (2>_z^1/2) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of 2>_z^1/2 = k M_w^v were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on M_w was established to be [η] = 5.3 × 10⁻²M_w^0.61 (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII–IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50–400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.