期刊论文详细信息
Journal of Applied Biological Sciences
Investigation on Toxoplasma gondii in Surface and Drinking Water Samples from Amasya by Nested Polymerase Chain Reaction
Zeynep KOLOREN1  Elif DEMİREL1 
关键词: Toxoplasma gondii;    Nested PCR;    Surface water;    Black Sea;   
DOI  :  
学科分类:生物科学(综合)
来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente
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【 摘 要 】

OccurenceofToxoplasmagondiiwasinvestigatedinsurfaceanddrinkingwatersamplescollectedfromAmasyabynestedPCR.Total120surfacewatersamplesand20drinkingwatersampleswereflocculatedbyAluminumSulfateandtheywerepurifiedsucrose-gradient.AllofthewatersampleswereusedforDNAextractionandthen,nestedPCRwereperformedforthem.While48outof120watersamples(40%)werefoundpositiveforT.gondii,therewerenoanycontaminationin20drinkingwatersamplesforToxoplasma.ThewatersamplestakenfromTersakan(Suluova),Tersakan(Karasu),TersakanlastandYesilırmak(Tasova)stationswerepositiveforToxoplasma.ThisstudyisfirstreportoninvestigationT.gondiiinAmasyabyusingNestedPCR.AmasyaProvincehasextensiveagricultureandanimalhusbandryandalso,ithasinsufficientwastewatertreatmentplants.SinceRiverYesilırmakisusedforagriculturalterrainirrigation,thisstudyisimportantinmaintainingpublichealthofthisregion.

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