Development of a test system for detecting bovine adenovirus DNA based on polymerase chain reaction

International Scientific Conference «AGRITECH-2019: Agribusiness, Environmental Engineering and Biotechnologies»

农业科学;生态环境科学;生物科学

Shemel'Kova, G.O.^1 ; Yuzhakov, A.G.^2 ; Zaberezhny, A.D.^2 ; Shemel'Kov, E.V.^2 ; Aliper, T.I.^2

Vetbiochem, Gamaleya st., 16, Moscow

123098, Russia^1

Federal State Budget Scientific Institution Federal Scientific Centre VIEV (FSC VIEV), 24/1, Ryazanskiy prospect, Moscow

109428, Russia^2

关键词: Nested PCR;    Test systems;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/315/4/042027/pdf
DOI  :  10.1088/1755-1315/315/4/042027

来源: IOP

【 摘 要 】

The paper presents the results of the development of polymerase chain reaction (PCR) test systems for detecting the DNA of bovine adenovirus. Due the large number of classified types of bovine adenoviruses included in two separate genera of Mastadenovirus and Atadenovirus, a nested PCR variant was chosen when creating a test system using a common external pair of primers and internal primers specific for the Mastadenovirus and Atadenovirus genera. In the study of field samples from calves with a characteristic clinical picture using the developed test system, the circulation of different strains of bovine adenovirus, which are representatives of these genera, was established. Selective studies showed the presence of 1, 3, 6, and 8 subtypes of bovine adenovirus among positive samples.