Genipa americana and Ageratina anisochroma, two new hosts of Candidatus Phytoplasma asteris in Costa Rica

Australasian Plant Disease Notes

Mauricio Montero-Astúa¹  William Villalobos² 

[1] Escuela de Agronomía, UCR;Universidad de Costa Rica (UCR)

关键词: Nested PCR;    Real-time LAMP;    Jagua tree;    Guaitil tree;   

DOI  :  10.1007/s13314-018-0316-3

学科分类：农业科学（综合）

来源: Springer

【 摘 要 】

We report two new plant species hosts for Candidatus Phytoplasma asteris: Genipa americana (Rubiaceae) and Ageratina anisochroma (Asteraceae). Phytoplasma infections were detected by real-time loop-mediated isothermal amplification and nested PCR. Consensus sequences from both hosts share 99% identity to the 16SrI-B subgroup using BLAST; however, potential new subgroups are suggested due to unique RFLP patterns of the 16S rDNA F2nR2 fragment.

【 授权许可】

CC BY