Diagnostic value of nested-PCR for identification of Malassezia species in dandruff

1st International Conference on Tropical Medicine and Infectious Diseases (ICTROMI) Faculty of Medicine Universitas Sumatera Utara

Jusuf, N.K.^1 ; Nasution, T.A.^2 ; Ullyana, S.^1

Dermatology and Venerology Department, Faculty of Medicine, Universitas Sumatera Utara, Kampus USU, Jl. Dr. Mansur No.5, Medan

20155, Indonesia^1

Microbiology Department, Faculty of Medicine, Universitas Sumatera Utara, Kampus USU, Jl. Dr. Mansur No.5, Medan

20155, Indonesia^2

关键词: Diagnostic tests;    Diagnostic value;    Gold standards;    High sensitivity;    Identification method;    Negative predictive value;    Nested PCR;    Positive predictive values;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/125/1/012050/pdf
DOI  :  10.1088/1755-1315/125/1/012050

来源: IOP

【 摘 要 】

Dandruff or pityriasis simplex is a condition of abnormal occurrence of formation of yellowish white scales from the scalp. Many factors play a role in the pathogenesis of dandruff, i.e.colonization of Malassezia species. Examination of Malassezia species previously done by culture as the gold standard. However, there are various difficulties in doing the culture. Identification method with anested-polymerase chain reaction (nested-PCR) is expected to provide quickly and easily detected. This study aimedto determine the diagnostic value of nested-PCR in the identification of Malassezia species in dandruff. From 21 subjects, scales from the scalp were taken and sent to the laboratory for nested-PCR identification. Statistical analysis of diagnostic test carried out to determine sensitivity, specificity, positive predictive value, and negative predictive value. The results showed nested-PCR detected 10 sample (47.6%) positive for Malassezia species consist of M. sympodialis (23.8%); M. slooffiae (9.5%); M. furfur (4.8%); M. globosa and M. furfur (4.8%); and M. restricta and M. sympodialis (4.8%). Detection of Malassezia species by nested-PCR has 100% in sensitivity whereas the specificity was 55%. Nested-PCR test has high sensitivity. Therefore nested-PCR may be considered for a faster and simpler alternative examination in identification for Malassezia species in dandruff.