Switching assay as a novel approach for specific antigen- antibody interaction analysis using magnetic nanoparticles

3rd International School and Conference on Optoelectronics, Photonics, Engineering and Nanostructures (Saint Petersburg OPEN 2016)

Parr, M.^1 ; Illarionov, R.^2 ; Marchenko, Y.^1 ; Yakovleva, L.^3 ; Nikolaev, B.^3 ; Ischenko, A.^3 ; Shevtsov, M.^4

Department of Physics, Saint-Petersburg State University, Saint-Petersburg

199034, Russia^1

Department of Chemical and Biotechnology, Saint-Petersburg State Institute of Technology, Saint-Petersburg

190013, Russia^2

Research Institute of Highly Pure Biopreparations, Saint-Petersburg

197110, Russia^3

Institute of Cytology, Russian Academy of Sciences (RAS), Saint-Petersburg

194064, Russia^4

关键词: Antigen-antibody interaction;    Biological fluids;    Diagnostic value;    Heat shock protein;    High sensitivity;    Intracellular proteins;    Magnetic iron oxide nanoparticles;    Magnetic nano-particles;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/741/1/012062/pdf
DOI  :  10.1088/1742-6596/741/1/012062

来源: IOP

【 摘 要 】

Switching assay was applied for the detection of antigen-antibody interaction between 70-kDa heat shock protein (Hsp70) and anti-Hsp70 monoclonal antibodies in water solutions using conjugates with magnetic iron oxide nanoparticles (MNPs). Hsp70 is a ubiquitous intracellular protein that plays a crucial role in cancerogenesis and many other pathologies. Detection of the Hsp70 level in the biological fluids might have a prognostic and diagnostic value in clinic. The developed switch assay for the detection of Hsp70 demonstrated high sensitivity for antigen-antibody interaction analysis thus proving its potential for further preclinical and clinical studies.