Two kinds of anti-ricin antibody and ricin interaction evaluated by biosensor based on imaging ellipsometry

THIN SOLID FILMS

卷:519

Article; Proceedings Paper

Niu, Yu^1,2  Zhuang, Jie³  Liu, Li¹  Yan, Xiyun³  Jin, Gang¹ 

[1] Chinese Acad Sci, NML, Inst Mech, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China

关键词: Ricin detection;    Antigen-antibody interaction;    Biosensor;    Imaging ellipsometry;   

DOI  :  10.1016/j.tsf.2010.12.054

来源: Elsevier

【 摘 要 】

In order to meet the requirements of antibody comparison and evaluation, the optimization for the surface modification, ligand immobilization, unspecific blocking and ligand receptor interaction condition is introduced to the biosensor based on imaging ellipsometry (BIE), so that it may serve as an antibody screening platform to evaluate antibody antigen interactions. Two kinds of anti-ricin antibody named pVHHS1 and 5S1R are immobilized in a patterned array format on the silicon surface modified with succinic anhydride, respectively, to form a sensing surface. Ricin and its similar toxin abrin as the reference in solution are delivered, respectively, on the patterned sensing surface for anti-ricin antibody and ricin interaction. The limit of detection value of ricin detected by pVHHS1 and 5S1R is obtained as 1 mu g/ml and 5 ng/ml, respectively. Results show that pVHHS1 and 5S1R specifically bind with ricin but not with abrin, which is in accordance with ELISA's result. (C) 2010 Elsevier B.V. All rights reserved.

【 授权许可】

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