Membrane materials for storing biological samples intended for comparative nanotoxicological testing

3rd International Youth Conference on Interdisciplinary Problems of Nanotechnology, Biomedicine and Nanotoxicology

物理学;医药卫生;生态环境科学

Metelkin, A.^1 ; Kuznetsov, D.^1 ; Kolesnikov, E.^1 ; Chuprunov, K.^1 ; Kondakov, S.^1,2 ; Osipov, A.^1,2 ; Samsonova, J.^1,2

National Research Technological University mISiS, 2, Leninsky Ave., Moscow

119049, Russia^1

M.V. Lomonosov Moscow State University, 1-3 Lenin Hills, Moscow

119991, Russia^2

关键词: Biological fluids;    Biological samples;    Dried blood spots;    FTIR spectroscopy;    Membrane material;    Sampling systems;    Structural fibers;    Wetting property;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/98/1/012019/pdf
DOI  :  10.1088/1757-899X/98/1/012019

来源: IOP

【 摘 要 】

The study is aimed at identifying the samples of most promising membrane materials for storing dry specimens of biological fluids (Dried Blood Spots, DBS technology). Existing sampling systems using cellulose fiber filter paper have a number of drawbacks such as uneven distribution of the sample spot, dependence of the spot spreading area on the individual biosample properties, incomplete washing-off of the sample due to partially inconvertible sorption of blood components on cellulose fibers, etc. Samples of membrane materials based on cellulose, polymers and glass fiber with applied biosamples were studied using methods of scanning electron microscopy, FT-IR spectroscopy and surface-wetting measurement. It was discovered that cellulose-based membrane materials sorb components of biological fluids inside their structure, while membranes based on glass fiber display almost no interaction with the samples and biological fluid components dry to films in the membrane pores between the structural fibers. This characteristic, together with the fact that membrane materials based on glass fiber possess sufficient strength, high wetting properties and good storage capacity, attests them as promising material for dry samples of biological fluids storage systems.