Development of square-wave adsorptive stripping voltammetric method for determination of acebutolol in pharmaceutical formulations and biological fluids

Chemistry Central Journal

Ali F Al-Ghamdi²  Mohamed M Hefnawy³  Abdulrahman A Al-Majed³  Fatallah F Belal¹ 

[1] Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt

[2] Department of Chemistry, College of Science, Taibah University, P.O. Box 344, Al-Madinah Al-Munawarah, Saudi Arabia

[3] Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia

关键词: Biological fluids;    Pharmaceutical formulations;    Adsorptive stripping voltammetry;    Square wave voltammetry;    Acebutolol;   

Others  :  788377
DOI  :  10.1186/1752-153X-6-15

 received in 2011-11-21, accepted in 2012-02-21,  发布年份 2012

【 摘 要 】

A validated simple, rapid, sensitive and specific square-wave voltammetric technique is described for the determination of acebutolol (AC) following its accumulation onto a hanging mercury drop electrode in a Britton-Robinson universal buffer of pH 7.5. The optimal procedural conditions were: accumulation potential E_acc = - 0.8 V versus Ag/AgCl/KCl, accumulation duration t_acc = 30 s, pulse-amplitude = 70 mV, scan rate = 100 mV/s, frequency = 30 Hz, surface area of the working electrode = 0.6 mm² and the convection rate = 2000 rpm. Under these optimized conditions, the adsorptive stripping voltammetry (AdSV) peak current was proportional over the concentration range 5 × 10^-7 - 6 × 10^-6 M (r = 0.999). Recoveries for acebutolol from human plasma and urine were in the range 97-103% and 96-104% respectively. The method proved to be precise (intra-day precision expressed as %RSD in human plasma ranged from 2.9 - 3.2% and inter-day precision expressed as %RSD ranged from 3.4 - 3.8%) and accurate (intra-day accuracies expressed as % error in human urine ranged from -3.3 - 2.8% and inter-day accuracies ranged from -3.3 - 1.7%). The limit of quantitation (LOQ) and limit of detection (LOD) for acebutolol were 1.7 × 10^-7 and 5 × 10^-7 M, respectively. Possible interferences by substances usually present in the pharmaceutical formulations were investigated with a mean recovery of 101.6 ± 0.64%. Results of the developed square-wave adsorptive stripping voltammetry (SW-AdSV) method were comparable with those obtained by reference analytical method.

【 授权许可】

   
2012 Al-Ghamdi et al

【 图 表 】

Scheme 1

Figure 1.

Figure 2.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

Figure 8.

Figure 9.

Figure 10.

Figure 11.

【 参考文献 】

• [1]Parfitt K (Ed): Martindale: The Complete Drug Reference. 33rd edition. Pharmaceutical Press: London, UK; 2002.
• [2]Delamoye M, Duverneuil C, Paraire F, de Mazancourt P, Alvarez JC: Simultaneous determination of thirteen beta-blockers and one metabolite by gradient high-performance liquid chromatography with photodiode-array UV detection. Forensic Sci Int 2004, 141(1):23-31.
• [3]Saarinen MT, Sirèn H, Riekkola ML: Screening and determination of β-blockers, narcotic analgesics and stimulants in urine by high-performance liquid chromatography with column switching. J Chromatog B Biomed Sci Appl 1995, 664(2):341-346.
• [4]Pelander A, Ojanpera I, Laks S, Rasanen I, Vuori E: Toxicological screening with formula-based metabolite identification by liquid Chromatography/time-of-flight mass spectrometry. Anal Chem 2003, 75(21):5710-5718.
• [5]Brunelli C, Bicchi C, Di Stilo A, Salomone A, Vincenti M: High-speed gas chromatography in doping control: fast-GC and fast-GC/MS determination of beta-adrenoceptor ligands and diuretics. J Sep Sci 2006, 29(18):2765-2771.
• [6]Bai X, You TY, Sun H, Yang X, Wang E: Determination of three-β-blockers by capillary electrophoresis with end column electrochemical detection. Electroanalysis 2000, 12(8):1379-1382.
• [7]Sastry CS, Rao SG, Y Naidu P, Srinivas KR: New spectrophotometric method for the determination of some drugs with iodine and wool fast blue BL. Talanta 1998, 45(6):1227-1234.
• [8]Abdelatef HE, El-Henawee MM, El-Sayed HM, Ayad HM: Spectrophotometric and spectrofluorimetric methods for analysis of acyclovir and acebutolol hydrochloride. Spectrochim Acta A Mol Biomol Spectrosc 2006, 65(3-4):997-999.
• [9]El-Gindy A, Ashour A, Abdel-Fattah L, Shabana MM: First derivative spectrophotometric, TLC-densitometric, and HPLC determination of acebutolol HCL in presence of its acid-induced degradation product. J Pharm Biomed Anal 2001, 24(4):527-534.
• [10]el-Walily AF: Analysis of nifedipine-acebutolol hydrochloride binary combination in tablets using UV-derivative spectroscopy, capillary gas chromatography and high performance liquid chromatography. J Pharm Biomed Anal 1997, 16(1):21-30.
• [11]Sanbe H, Haginaka J: Restricted access media-molecularly imprinted polymer for propranolol and its application to direct injection analysis of β-blockers in biological fluids. Analyst 2003, 128(6):593-597.
• [12]Pospìšilová M, Kavalìrová A, Polášek M: Assay of acebutolol in pharmaceuticals by analytical capillary isotachophoresis. J Chromatog A 2005, 1081(1):72-76.
• [13]Phuong NT, Lee KA, Kim KH, Choi JK, Kim JM, Kang JS: Determination of stability constants of the inclusion complexes of beta-blockers in heptakis (2,3-dimethyl-6-sulfato)-beta-cyclodextrin. Arch Pharm Res 2004, 27(12):1290-1294.
• [14]Catarcini P, Fanali S, Presutti C, Dacquarica I, Gasparrini F: Evaluation of teicoplanin chiral stationary phases of 3.5 and 5 microm inside diameter silica microparticles by polar-organic mode capillary electrochromatography. Electrophoresis 2003, 24(17):3000-3005.
• [15]Schmid MG, Gugirz G, Kilar F: Stereoselective interaction of drug enantiomers with human serum transferrin in capillary zone electrophoresis (II). Electrophoresis 1998, 19(2):282-287.
• [16]Peterson AG, Foley JP: Influence of the inorganic counterion on the chiral micellar electrokinetic separation of basic drugs using the surfactant N-dodecoxycarbonylvaline. J Chromatogr B Biomed Sci Appl 1997, 695(1):131-145.
• [17]Nilsson S, Schweitz L, Petersson M: Three approaches to enantiomer separation of beta-adrenergic antagonists by capillary electrochromatography. Electrophoresis 1997, 18(6):884-890.
• [18]Kafková B, Bosáková Z, Tesarová E, Coufal P: Chiral separation of beta-adrenergic antagonists, profen non-steroidal anti-inflammatory drugs and chlorophenoxypropionic acid herbicides using teicoplanin as the chiral selector in capillary liquid chromatography. J Chromatog A 2005, 1088(1-2):82-93.
• [19]Desiderio C, Aturki Z, Fanali S: Use of vancomycin silica stationary phase in packed capillary electrochromatography I. Enantiomer separation of basic compounds. Electrophoresis 2001, 22(3):535-543.
• [20]Honetschlägerová-Vadinská M, Srkalová S, Bosáková Z, Coufal P, Tesarová E: Comparison of enantioselective HPLC separation of structurally diverse compounds on chiral stationary phases with different teicoplanin coverage and distinct linkage chemistry. J Sep Sci 2009, 32(10):1704-1711.
• [21]Al-Omar MA: Stereoselective HPLC assay of acebutolol enantiomers with fluorescence detection and its application to pharmacokinetic study. World Appl Sci J 2010, 8(11):1309-1316.
• [22]Bosáková Z, Curínová E, Tesarová E: Comparison of vancomycin-based stationary phases with different chiral selector coverage for enantioselective separation of selected drugs in high-performance liquid chromatography. J Chromatog A 2005, 1088(1-2):94-103.
• [23]Jiang H, Randlett C, Junga H, Jiang X, Ji QS: Using supported liquid extraction together with cellobiohydrolase chiral stationary phases-based liquid chromatography with tandem mass spectrometry for enantioselective determination of acebutolol and its active metabolite diacetolol in spiked human plasma. J Chromatog B Anal Techn Biomed Life Sci 2009, 877(3):173-180.
• [24]Kim KH, Choi PW, Hong SP, Kim HJ: Chiral separation of beta-blockers after derivatization with (-)-menthyl chloroformate by reversed-phase high performance liquid chromatography. Arch Pharml Res 1999, 22(6):608-613.
• [25]Szymura-Oleksiak J, Walczak M, Bojarski J, Aboul-Enein HY: Enantioselective high performance liquid chromatographic assay of acebutolol and its active metabolite diacetolol in human serum. Chirality 1999, 11(4):267-271.
• [26]Wang J (Ed): Analytical Electrochemistry. 2nd edition. Wiley-VCH, New York; 2002.
• [27]Cunningham L, Freiser H: On-selective electrodes for some β-adrenergic and calcium blockers. Anal Chim Acta 1984, 156:157-162.
• [28]Mostafa GA, Hefnawy MM, Al-Majed A: PVC membrane sensors for potentiometric determination of acebutolol. Sensors 2007, 7:3272-3286.
• [29]Al-Ghamdi HA, Al-Ghmdi AF, Al-Omar MA: Electrochemical Studies and Square-Wave Adsorptive Stripping Voltammetry of Spironolactone Drug. Anal Lett 2008, 41(1):90-103.
• [30]Laviron E: A multilayer model for the study of space distributed redox modified electrodes: Part I. Description and discussion of the model. J Elec anal Chem 1980, 112:1-9.
• [31]Yilmaz S: Adsorptive stripping voltammetric determination of zopiclone in tablet dosage forms and human urine. Colloids Surf B Biointerfaces 2009, 71(1):79-83.