Poly(L-lactide-co-glycolide) thin films can act as autologous cell carriers for skin tissue engineering

Cellular & Molecular Biology Letters

Justyna Drukala²  Elzbieta Pamula¹  Malgorzata Krok¹  Julia Borowczyk²  Aleksandra Zuber²  Zbigniew Madeja²  Eliza Zimolag² 

[1] Department of Biomaterials, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, Poland$$;Laboratory of Cell & Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland$$

关键词: Burns;    Skin regeneration;    Wound healing;    Keratinocytes;    Fibroblasts;    Biomaterials;    PLGA;    Tissue engineering;   

DOI  :  10.2478/s11658-014-0197-1

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.

【 授权许可】

Unknown