The ultrastructural surface morphology of oral cancer cells and keratinocytes after exposure to chitosan

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Fatimah^1 ; Sarsito, A.S.^1 ; Wimardhani, Y.S.^1

Department of Oral Medicine, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

关键词: Cell lines;    Cytotoxic effects;    Keratinocytes;    Low molecular weight chitosans;    Oral cancer cells;    Oral squamous cell carcinomata;    Primary cells;    Scanning electron micrographs;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012064/pdf
DOI  :  10.1088/1742-6596/884/1/012064

学科分类：卫生学

来源: IOP

【 摘 要 】

Low-molecular-weight chitosan (LMWC) has the same selective cytotoxic effects on oral cancer cells as cisplatin. The cell deaths caused by the anticancer characteristics of chitosan show that apoptosis is not the death pathway of the primary cells involved. The interactions between LMWC and the cells need to be explored. The objective of this study was to compare the ultrastructural morphology of oral Squamous Cell Carcinoma (SCC Ca)-922 and noncancer keratinocyte HaCaT cell lines after exposure to LMWC and cisplatin. The cells were treated with LMWC and cisplatin, and their ultrastructural morphology was analyzed using scanning electron micrographs. Features of early apoptosis, seen as the loss of microvilli, were detected in the LMWC-exposed Ca9-22 cells, and there was a material surrounding the cells. In contrast, the LMWC-exposed HaCaT cells showed no changes related to apoptosis. The results were the opposite when cisplatin was used. This study confirms that there are differences in the ultrastructural surface morphology of LMWC-exposed and cisplatin-exposed oral cancer cells and keratinocytes that could be correlated with their biological activity.