Biological characteristics of stem cells derived from burned skin—a comparative study with umbilical cord stem cells

Stem Cell Research & Therapy

Yufei Andy Chen¹  Alexandra Parousis¹  Christopher Auger¹  Andrea-Kaye Datu¹  Ayesha Aijaz¹  Marc G. Jeschke²  Saeid Amini-Nik³  Reinhard Dolp⁴  Gertraud Eylert⁵ 

[1] Sunnybrook Research Institute, Toronto, Canada;Sunnybrook Research Institute, Toronto, Canada;Department of Immunology, Ross Tilley Burn Centre, Sunnybrook Health Sciences Centre, 2075 Bayview Ave., M4N 3M5, Toronto, ON, Canada;Division of Plastic and Reconstructive Surgery, Department of Surgery, Faculty of Medicine, University of Toronto, Toronto, Canada;Ross Tilley Burn Centre, Sunnybrook Health Science Centre, Toronto, Canada;Sunnybrook Research Institute, Toronto, Canada;Department of Laboratory Medicine and Pathobiology (LMP), University of Toronto, Toronto, Canada;SGS Harrison Research Laboratories, SGS North America, New York Metropolitan Area, Union, NJ, USA;Sunnybrook Research Institute, Toronto, Canada;Department of Psychiatry, Queen’s University, Kingston, Canada;Institute of Medical Science, University of Toronto, Ontario, Canada;Sunnybrook Research Institute, Toronto, Canada;Institute of Medical Science, University of Toronto, Ontario, Canada;Division of Plastic, Aesthetic and Reconstructive Surgery, Medical University of Graz, Graz, Austria;

关键词: Mesenchymal stromal/stem cells;    • Burn-derived mesenchymal stromal/stem cells;    • Umbilical cord mesenchymal stromal/stem cells;    Cell Therapy;    Skin regeneration;    Wound healing;    Burn(s);    Cell biologic function;   

DOI  :  10.1186/s13287-021-02140-z

来源: Springer

【 摘 要 】

IntroductionBurned human skin, which is routinely excised and discarded, contains viable mesenchymal stromal/stem cells (burn-derived mesenchymal stromal/stem cells; BD-MSCs). These cells show promising potential to enable and aid wound regeneration. However, little is known about their cell characteristics and biological function.ObjectivesThis study had two aims: first, to assess critical and cellular characteristics of BD-MSCs and, second, to compare those results with multipotent well-characterized MSCs from Wharton’s jelly of human umbilical cords (umbilical cord mesenchymal stromal/stem cells, UC-MSCs).MethodsBD- and UC-MSCs were compared using immunophenotyping, multi-lineage differentiation, seahorse analysis for glycolytic and mitochondrial function, immune surface markers, and cell secretion profile assays.ResultsWhen compared to UC-MSCs, BD-MSCs demonstrated a lower mesenchymal differentiation capacity and altered inflammatory cytokine secretomes at baseline and after stimulation with lipopolysaccharides. No significant differences were found in population doubling time, colony formation, cell proliferation cell cycle, production of reactive oxygen species, glycolytic and mitochondrial function, and in the expression of major histocompatibility complex I and II and toll-like receptor (TLR).Importance, translationThis study reveals valuable insights about MSCs obtained from burned skin and show comparable cellular characteristics with UC-MSCs, highlighting their potentials in cell therapy and skin regeneration.

【 授权许可】

CC BY