Chondrogenic induction of mesenchymal stromal/stem cells from Wharton’s jelly embedded in alginate hydrogel and without added growth factor: an alternative stem cell source for cartilage tissue engineering

Stem Cell Research & Therapy

Céline Huselstein¹  Danièle Bensoussan¹  Jean-François Stoltz¹  Christel Henrionnet¹  Astrid Pinzano¹  Hao Yu¹  Léonore Leger¹  Naceur Charif¹  Jessica Schiavi¹  Loïc Reppel¹ 

[1] Fédération de Recherche 3209, Bioingénierie Moléculaire Cellulaire et Thérapeutique, Vandœuvre-lès-Nancy, 54500, France

关键词: Wharton’s jelly;    Mesenchymal stromal/stem cells;    Cartilage tissue engineering;    Chondrogenic differentiation;    Alginate/hyaluronic acid hydrogel;   

Others  :  1235186
DOI  :  10.1186/s13287-015-0263-2

 received in 2015-05-12, accepted in 2015-12-08,  发布年份 2015

【 摘 要 】

Background

Due to their intrinsic properties, stem cells are promising tools for new developments in tissue engineering and particularly for cartilage tissue regeneration. Although mesenchymal stromal/stem cells from bone marrow (BM-MSC) have long been the most used stem cell source in cartilage tissue engineering, they have certain limits. Thanks to their properties such as low immunogenicity and particularly chondrogenic differentiation potential, mesenchymal stromal/stem cells from Wharton’s jelly (WJ-MSC) promise to be an interesting source of MSC for cartilage tissue engineering.

Methods

In this study, we propose to evaluate chondrogenic potential of WJ-MSC embedded in alginate/hyaluronic acid hydrogel over 28 days. Hydrogels were constructed by the original spraying method. Our main objective was to evaluate chondrogenic differentiation of WJ-MSC on three-dimensional scaffolds, without adding growth factors, at transcript and protein levels. We compared the results to those obtained from standard BM-MSC.

Results

After 3 days of culture, WJ-MSC seemed to be adapted to their new three-dimensional environment without any detectable damage. From day 14 and up to 28 days, the proportion of WJ-MSC CD73 ⁺ , CD90 ⁺ , CD105 ⁺and CD166 ⁺decreased significantly compared to monolayer marker expression. Moreover, WJ-MSC and BM-MSC showed different phenotype profiles. After 28 days of scaffold culture, our results showed strong upregulation of cartilage-specific transcript expression. WJ-MSC exhibited greater type II collagen synthesis than BM-MSC at both transcript and protein levels. Furthermore, our work highlighted a relevant result showing that WJ-MSC expressed Runx2 and type X collagen at lower levels than BM-MSC.

Conclusions

Once seeded in the hydrogel scaffold, WJ-MSC and BM-MSC have different profiles of chondrogenic differentiation at both the phenotypic level and matrix synthesis. After 4 weeks, WJ-MSC, embedded in a three-dimensional environment, were able to adapt to their environment and express specific cartilage-related genes and matrix proteins. Today, WJ-MSC represent a real alternative source of stem cells for cartilage tissue engineering.

【 授权许可】

   
2015 Reppel et al.

【 图 表 】

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