Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic differentiation

Stem Cell Research & Therapy

Jean-Marc Virion¹  Philippe Guerci²  Sébastien Hupont³  Jean-François Stoltz⁴  Léonore Avercenc-Léger⁴  Danièle Bensoussan⁴  Céline Huselstein⁴  Jacques Magdalou⁴  Ghislaine Cauchois⁴  Loïc Reppel⁴  Rachid Rahouadj⁵ 

[1] CHRU de Nancy, Epidémiologie et Evaluation Cliniques;CHRU de Nancy, Maternité Régionale Universitaire, Département d’Anesthésie-Réanimation;FR3209 CNRS BMCT – Bio-Ingénierie Moléculaire Cellulaire et Thérapeutique, Faculté de Médecine;UMR 7365 CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Biopôle de l’Université de Lorraine, Campus biologie-santé, Faculté de Médecine;Université de Lorraine;

关键词: Chondrogenic differentiation;    Mesenchymal stromal cells;    Obstetric factors;    Proliferation;    Umbilical cord;    Wharton’s jelly;   

DOI  :  10.1186/s13287-017-0609-z

来源: DOAJ

【 摘 要 】

Abstract Background The umbilical cord is becoming a notable alternative to bone marrow (BM) as a source of mesenchymal stromal cells (MSC). Although age-dependent variations in BM-MSC are well described, less data are available for MSC isolated from Wharton’s jelly (WJ-MSC). We initiated a study to identify whether obstetric factors influenced MSC properties. We aimed to evaluate the correlation between a large number of obstetric factors collected during pregnancy and until peripartum (related to the mother, the labor and delivery, and the newborn) with WJ-MSC proliferation and chondrogenic differentiation parameters. Methods Correlations were made between 27 obstetric factors and 8 biological indicators including doubling time at passage (P)1 and P2, the percentage of proteoglycans and collagens, and the relative transcriptional expression of Sox-9, aggrecans, and total type 2 collagen (Coll2T). Results Amongst the obstetric factors considered, birth weight, the number of amenorrhea weeks, placental weight, normal pregnancy, and the absence of preeclampsia were identified as relevant factors for cell expansion, using multivariate linear regression analysis. Since all the above parameters are related to term, we concluded that WJ-MSC from healthy, full-term infants exhibit greater proliferation capacity. As for chondrogenesis, we also observed that obstetric factors influencing proliferation seemed beneficial, with no negative impact on MSC differentiation. Conclusions Awareness of obstetric factors influencing the proliferation and/or differentiation of WJ-MSC will make it possible to define criteria for collecting optimal umbilical cords with the aim of decreasing the variability of WJ-MSC batches produced for clinical use in cell and tissue engineering.

【 授权许可】

Unknown